![]() ![]() The intensity of the light is than measure compared to a calibration curve or standard in order to give a quantitative result. ![]() As the UV or IR light passes through the sample it is absorbed or reflected depending on the molecules present. This allows photons to readily pass through the sample. The sample is pressed into a flat crystalline disc using a diamond tip. dissolution to determine the amount of drug released in the medium.Ī spectrophotometer is an instrument that measures the light photons passing through a sample. You could use UV-Vis in association with other tests e.g. FTIR and UV-Vis are primarily used for identification. UV-Vis can be an appropriate method for identifying an API or excipient. As such, each product must have an associated method for chemical analysis which is performed routinely on every batch. It is a regulatory requirement defined within every pharmacopoeia that products, both sterile and non-sterile, must be assessed for their chemical quality. UV-Vis is often used in HPLC for analysis of the separated mixture.Īssessing the constituent parts of a finished pharmaceutical product, raw material or API is an essential stage in determining product safety. This profile allows quantitative determination of analytes in a sample. Depending on the atomic composition of each compound, each will have its own absorption (or reflection) profile. Atoms and molecules absorb the energy from the light and under-go electronic transitions. FTIR (Fourier-Transform Infrared Spectroscopy) uses infrared light to the same purpose. UV (Ultra-violet) spectrophotometers use visible light to determine the concentration of chemicals in a mixture. ![]()
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